化学
烯丙胺
溶解
细胞毒性
盐酸阿霉素
核酸
右旋糖酐
阿霉素
DNA
磷酸盐
劈理(地质)
盐酸盐
生物物理学
化学工程
核化学
色谱法
体外
有机化学
聚电解质
聚合物
生物化学
医学
工程类
化疗
岩土工程
断裂(地质)
生物
外科
作者
Fujian Huang,Wensheng Liao,Yang Sung Sohn,Rachel Nechushtai,Chun Hua Lu,Itamar Willner
摘要
A method to assemble light-responsive or pH-responsive microcapsules loaded with different loads (tetramethylrhodamine-modified dextran, TMR-D; microperoxidase-11, MP-11; CdSe/ZnS quantum dots; or doxorubicin-modified dextran, DOX-D) is described. The method is based on the layer-by-layer deposition of sequence-specific nucleic acids on poly(allylamine hydrochloride)-functionalized CaCO3 core microparticles, loaded with the different loads, that after the dissolution of the core particles with EDTA yields the stimuli-responsive microcapsules that include the respective loads. The light-responsive microcapsules are composed of photocleavable o-nitrobenzyl-phosphate-modified DNA shells, and the pH-responsive microcapsules are made of a cytosine-rich layer cross-linked by nucleic acid bridges. Irradiating the o-nitrobenzyl phosphate-functionalized microcapsules, λ = 365 nm, or subjecting the pH-responsive microcapsules to pH = 5.0, results in the cleavage of the microcapsule shells and the release of the loads. Preliminary studies address the cytotoxicity of the DOX-D-loaded microcapsules toward MDA-MB-231 breast cancer cells and normal MCF-10A breast epithelial cells. Selective cytotoxicity of the DOX-D-loaded microcapsules toward cancer cells is demonstrated.
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