Screen printed electrodes on interfacial Pt–CuO/carbon nanofiber functional ink for real-time qualification of cell released hydrogen peroxide

Screen printed electrode (SPE) on carbon-based inks exhibits promising applications in biosensing, environment protection and food safety. We report here a unique carbon-based material comprising Pt-CuO nanocrystal interfacially anchored on functionalized carbon nanofiber (Pt-CuO@FCNF) and its functional ink to build SPE for ultrasensitive detection of cell released H2O2. Pt-CuO@FCNF is fabricated using a one-pot and mass production method through direct pyrolysis of Pt and CuO precursors together with FCNF. FCNF with 1-D structure and high electrical conductivity can interfically anchor Pt-CuO nanocrystal, which synergically promotes rich active site and catalytic activity towards H2O2. Pt-CuO@FCNF exhibits a wide linear response of 0.4 μM-11 mM, a low detection limit of 17 nM, a fast response time of 1.0 s, and good selectivity. Eventually, Pt-CuO@FCNF SPE realizes real-time and ultrasensitive qualification of H2O2 released from both normal and cancer cells.