微流控
分析物
实验室晶片
炸薯条
纳米技术
可重用性
自动化
接口
实验室自动化
检出限
嵌入式系统
生物芯片
计算机科学
化学
计算机硬件
色谱法
工程类
材料科学
机械工程
程序设计语言
电信
软件
作者
Binyao Liu,Yunhai Cheng,Xiang Peng,Wen Yang,Xiangpeng Li,Xun Hu,Haihang Ye,Tingrui Pan
标识
DOI:10.1021/acs.analchem.3c05918
摘要
The ability to deploy decentralized laboratories with autonomous and reliable disease diagnosis holds the potential to deliver accessible healthcare services for public safety. While microfluidic technologies provide precise manipulation of small fluid volumes with improved assay performance, their limited automation and versatility confine them to laboratories. Herein, we report the utility of multicolor assay-on-a-chip processed by robotic operation (MACpro), to address this unmet need. The MACpro platform comprises a robot-microfluidic interface and an eye-in-hand module that provides flexible yet stable actions to execute tasks in a programmable manner, such as the precise manipulation of the microfluidic chip along with different paths. Notably, MACpro shows improved detection performance by integrating the microbead-based antibody immobilization with enhanced target recognition and multicolor sensing via Cu2+-catalyzed plasmonic etching of gold nanorods for rapid and sensitive analyte quantification. Using interferon-gamma as an example, we demonstrate that MACpro completes a sample-to-answer immunoassay within 30 min and achieves a 10-fold broader dynamic range and a 10-fold lower detection limit compared to standard enzyme-linked immunosorbent assays (0.66 vs 5.2 pg/mL). MACpro extends the applications beyond traditional laboratories and presents an automated solution to expand diagnostic capacity in diverse settings.
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