自噬
细胞生物学
免疫系统
生物
遗传学
细胞凋亡
作者
Enoch Lok Him Yuen,Alexandre Y Leary,Marion Clavel,Yasin Tümtaş,Azadeh Mohseni,Jierui Zhao,Lorenzo Picchianti,Mostafa Jamshidiha,Pooja Pandey,Cian Duggan,Ernesto Cota,Yasin Dagdas,Tolga O. Bozkurt
标识
DOI:10.1016/j.cub.2024.04.002
摘要
Plants rely on autophagy and membrane trafficking to tolerate stress, combat infections, and maintain cellular homeostasis. However, the molecular interplay between autophagy and membrane trafficking is poorly understood. Using an AI-assisted approach, we identified Rab3GAP-like (Rab3GAPL) as a key membrane trafficking node that controls plant autophagy negatively. Rab3GAPL suppresses autophagy by binding to ATG8, the core autophagy adaptor, and deactivating Rab8a, a small GTPase essential for autophagosome formation and defense-related secretion. Rab3GAPL reduces autophagic flux in three model plant species, suggesting that its negative regulatory role in autophagy is conserved in land plants. Beyond autophagy regulation, Rab3GAPL modulates focal immunity against the oomycete pathogen Phytophthora infestans by preventing defense-related secretion. Altogether, our results suggest that Rab3GAPL acts as a molecular rheostat to coordinate autophagic flux and defense-related secretion by restraining Rab8a-mediated trafficking. This unprecedented interplay between a RabGAP-Rab pair and ATG8 sheds new light on the intricate membrane transport mechanisms underlying plant autophagy and immunity.
科研通智能强力驱动
Strongly Powered by AbleSci AI