基因敲除
脉络丛
Cre重组酶
RNA干扰
生物
免疫系统
细胞生物学
掷骰子
基因
小RNA
中枢神经系统
神经科学
转基因
免疫学
转基因小鼠
核糖核酸
遗传学
作者
Yuwen Yang,Chen-Xing Qi,Lanxin Hu,Cheng Zheng,Xuhang Li,Wu Zheng,Yiyun Weng,Haiyan Lin
摘要
The choroid plexus (ChP) serves as a critical gateway for immune cell infiltration into the central nervous system (CNS) under both physiological and pathological conditions. Recent research has shown that regulating ChP activity may offer protection against CNS disorders. However, studying the biological function of the ChP without affecting other brain regions is challenging due to its delicate structure. This study presents a novel method for gene knockdown in ChP tissue using adeno-associated viruses (AAVs) or cyclization recombination enzyme (Cre) recombinase protein consisting of TAT sequence (CRE-TAT). The results demonstrate that after injecting AAV or CRE-TAT into the lateral ventricle, the fluorescence was exclusively concentrated in the ChP. Using this approach, the study successfully knocked down the adenosine A2A receptor (A2AR) in the ChP using RNA interference (RNAi) or Cre/locus of X-overP1 (Cre/LoxP) systems, and showed that this knockdown could alleviate the pathology of experimental autoimmune encephalomyelitis (EAE). This technique may have important implications for future research on the ChP's role in CNS disorders.
科研通智能强力驱动
Strongly Powered by AbleSci AI