材料科学
荧光寿命成像显微镜
荧光显微镜
显微镜
荧光
共轭体系
生物物理学
共焦显微镜
双光子激发显微术
膜
纳米技术
光学
化学
细胞生物学
生物
生物化学
物理
复合材料
聚合物
作者
Ji‐Yu Zhu,Alexander Mikhailovsky,Shichao Wei,Alex S. Moreland,Jakkarin Limwongyut,Nekane Guarrotxena,Guillermo C. Bazan
标识
DOI:10.1002/adfm.202305962
摘要
Abstract Conjugated oligoelectrolytes (COEs) comprise a class of cell‐membrane intercalating molecules that serve as effective optical reporters. However, little is known about the photophysical properties of COEs in biological environments such as buffers, cell membranes, and intracellular organelles, which is critical to optimize performance. Herein, how COE self‐assembly depends on the dielectric environment (polarity and ion content) is explored based on the representative molecule 6‐ring phenylenevinylene (PV) conjugated oligoelectrolyte (COE‐S6), and its optical properties within mammalian cells are subsequently studied. Two‐photon fluorescence lifetime imaging microscopy (FLIM), confocal laser scanning microscopy, and optical properties in solutions are brought together to obtain information about the location, accumulation, and characteristics of the local surroundings. FLIM imaging lifetime phasor plots, decays, and fluorescence spectra on stained mammalian cells provide evidence of successful COE‐S6 internalization via endocytosis. The fluorescence lifetime of COE‐S6 is identical when in A549 mammalian cells and in giant unilamellar vesicle model membranes, thereby providing a correlation between living system and artificial constructs.
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