马车
肺炎支原体
呼吸道感染
免疫学
流感嗜血杆菌
医学
肺炎
肺炎链球菌
嗜血杆菌
呼吸道
呼吸系统
生物
内科学
微生物学
抗生素
病理
细菌
遗传学
作者
Mischa H. Koenen,Ruben C.A. de Groot,Wouter A.A. de Steenhuijsen Piters,Mei Ling J N Chu,Kayleigh Arp,Raïza Hasrat,Adrianus C. J. M. de Bruijn,Silvia Estevão,Erhard van der Vries,Jeroen D. Langereis,Marianne Boes,Debby Bogaert,Annemarie M. C. van Rossum,Wendy W.J. Unger,Lilly M. Verhagen
出处
期刊:EBioMedicine
[Elsevier]
日期:2023-12-01
卷期号:98: 104868-104868
被引量:1
标识
DOI:10.1016/j.ebiom.2023.104868
摘要
BackgroundMycoplasma pneumoniae is a common cause of community-acquired pneumonia in school-aged children and can be preceded by asymptomatic carriage. However, its role in recurrent respiratory tract infections is unclear. We studied the prevalence of M.pneumoniae carriage in children with recurrent respiratory infections and identified associated factors.MethodsWe tested M.pneumoniae carriage by qPCR in children with recurrent infections and their healthy family members in a cross-sectional study. Serum and mucosal total and M.pneumoniae-specific antibody levels were measured by ELISA and nasopharyngeal microbiota composition was characterized by 16S-rRNA sequencing.FindingsPrevalence of M.pneumoniae carriage was higher in children with recurrent infections (68%) than their family members without infections (47% in siblings and 27% in parents). M.pneumoniae carriage among family members appeared to be associated with transmission within the household, likely originating from the affected child. In logistic regression corrected for age and multiple comparisons, IgA (OR 0.16 [0.06–0.37]) and total IgG deficiency (OR 0.15 [0.02–0.74]) were less prevalent in M.pneumoniae carriers (n = 78) compared to non-carriers (n = 36). In multivariable analysis, the nasopharyngeal microbiota of M.pneumoniae carriers had lower alpha diversity (OR 0.27 [0.09–0.67]) and a higher abundance of Haemophilus influenzae (OR 45.01 [2.74–1608.11]) compared to non-carriers.InterpretationM.pneumoniae carriage is highly prevalent in children with recurrent infections and carriers have a less diverse microbiota with an overrepresentation of disease-associated microbiota members compared to non-carriers. Given the high prevalence of M.pneumoniae carriage and the strong association with H. influenzae, we recommend appropriate antibiotic coverage of M.pneumoniae and H. influenzae in case of suspected pneumonia in children with recurrent respiratory tract infections or their family members.FundingWilhelmina Children’s Hospital Research Fund, ‘Christine Bader Stichting Irene KinderZiekenhuis’, Sophia Scientific Research Foundation, ESPID Fellowship funded by Seqirus, Hypatia Fellowship funded by Radboudumc and The Netherlands Organisation for Health Research and Development (ZonMW VENI grant to LM Verhagen).
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