DNMT3B型
DNA甲基化
甲基化
表观遗传学
发起人
生物
CpG站点
基因
遗传学
表观遗传学
体育锻炼的表观遗传学
RNA导向的DNA甲基化
甲基转移酶
DNA甲基转移酶
分子生物学
基因表达
作者
Jaqueline Loaeza-Loaeza,Angel Josué Cerecedo-Castillo,Hugo Alberto Rodríguez-Ruiz,Yaneth Castro‐Coronel,Oscar Del Moral‐Hernández,Félix Recillas‐Targa,Daniel Hernández-Sotelo
标识
DOI:10.1038/s41598-022-24186-6
摘要
DNA methylation is a key epigenetic modification to regulate gene expression in mammalian cells. Abnormal DNA methylation in gene promoters is common across human cancer types. DNMT3B is the main de novo methyltransferase enhanced in several primary tumors. How de novo methylation is established in genes related to cancer is poorly understood. CpG islands (CGIs), common sequences, and transcription factors (TFs) that interact with DNMT3B have been associated with abnormal de novo methylation. We initially identified cis elements associated with DNA methylation to investigate the contribution of DNMT3B overexpression to the deregulation of its possible target genes in an epithelial cell model. In a set of downregulated genes (n = 146) from HaCaT cells with DNMT3B overexpression, we found CGI, common sequences, and TFs Binding Sites that interact with DNMT3B (we called them P-down-3B). PPL1, VAV3, IRF1, and BRAF are P-down-3B genes that are downregulated and increased their methylation in DNMT3B presence. Together these findings suggest that methylated promoters aberrantly have some cis elements that could conduce de novo methylation by DNMT3B.
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