连接器
DNA折纸
生物素化
链霉亲和素
纳米技术
纳米结构
DNA
生物物理学
表皮生长因子
生物素
配体(生物化学)
材料科学
化学
生物
受体
生物化学
计算机科学
操作系统
作者
Carmen M. Domínguez,Miguel García‐Chamé,Ulrike Müller,Andreas Kraus,Klavdiya Gordiyenko,Ahmad M. Itani,Heiko Haschke,Peter Lanzerstorfer,Kersten S. Rabe,Christof M. Niemeyer
出处
期刊:Small
[Wiley]
日期:2022-08-07
卷期号:18 (35)
被引量:4
标识
DOI:10.1002/smll.202202704
摘要
News from an old acquaintance: The streptavidin (STV)-biotin binding system is frequently used for the decoration of DNA origami nanostructures (DON) to study biological systems. Here, a surprisingly high dynamic of the STV/DON interaction is reported, which is affected by the structure of the DNA linker system. Analysis of different mono- or bi-dentate linker architectures on DON with a novel high-speed atomic force microscope (HS-AFM) enabling acquisition times as short as 50 ms per frame gave detailed insights into the dynamics of the DON/STV interaction, revealing dwell times in the sub-100 millisecond range. The linker systems are also used to present biotinylated epidermal growth factor on DON to study the activation of the epidermal growth factor receptor signaling cascade in HeLa cells. The studies confirm that cellular activation correlated with the binding properties of linker-specific STV/DON interactions observed by HS-AFM. This work sheds more light on the commonly used STV/DON system and will help to further standardize the use of DNA nanostructures for the study of biological processes.
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