阶段(地层学)
脂质代谢
脂质积聚
化学
生产(经济)
单级
新陈代谢
生物化学
生物
工程类
古生物学
经济
宏观经济学
航空航天工程
作者
Yao Liu,Z. Z. Du,Penghui Wu,Lei Zhang
标识
DOI:10.1016/j.biortech.2024.130409
摘要
Traditionally, the time of maximum biomass concentration in stage I was the widely adopted stage conversion time in two-stage microalgae culture. This study challenges this conventional approach, demonstrating that the optimal stage conversion time in stage I is 72 h rather than 120 h for achieving maximum biomass concentration. A comparison of cell characteristics revealed that algal cells at 72 h exhibited better growth potential, leading to a higher biomass concentration after transfer to stage II and, consequently, increased lipid productivity. Moreover, the use of phosphorus repletion (5-fold) in stage II directed carbon flux toward biomass growth and lipid accumulation, thereby enhancing lipid productivity. By optimizing the stage conversion time and implementing phosphorus repletion, the mean lipid productivity of Auxenochlorella pyrenoidosa cultured under autotrophy-nitrogen starvation and autotrophy-high light conditions increased by 31 % and 60 %, respectively. This study underscores the importance of reevaluating the currently widely used stage conversion time.
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