葛根
化学
色谱法
大豆苷
萃取(化学)
葛根素
深共晶溶剂
大豆黄酮
共晶体系
有机化学
医学
替代医学
合金
病理
染料木素
内科学
作者
Bing Li,Pan Jiao,Cheng Tang
标识
DOI:10.1002/jssc.202300672
摘要
In this study, a deep eutectic solvent (DES) extraction combined with a magnetic bead ligand affinity analytical method was developed and used for α‐glucosidase inhibitor identification from Pueraria lobata . Several critical parameters affecting the analysis performance, including the type of DES, molar ratio, water amount, pH, salt concentration, and volume of DES, were investigated. The selected analytical sample preparation conditions were as follows. The composition of DES is choline chloride‐1,4‐butanediol (1:3), the water content is 40%, pH is 7.0 and the volume of extraction solution is 2 mL. The obtained sample extraction solution was analyzed directly using α‐glucosidase immobilized magnetic beads (GMBs). Three α‐glucosidase inhibitors in Pueraria lobata , including puerarin, daidzin, and daidzein, were identified. Luteolin was used as a positive control to evaluate the method's selectivity. Results showed it could selectively bond to the GMBs in the DES. As the affinity analysis was performed directly in a DES, the solution‐removing process could be avoided. The intra‐day and inter‐day precisions of the method are 5.21% and 6.38%, respectively. The solvent amount was 1/50–1/2000 of that used in traditional methods.
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