Within the frame of the current study, we assembled and annotated seven mitochondrial genomes: three of them for the fi rst time obtained for the Altai vole M. obscurus, four belong to the Eastern lineage of common vole M. arvalis.Obtained sequences enlarge amount of available genomic data for two sister vole species that creates the basis for further studies on phylogeny and mechanisms of adaptive mitochondrial DNA introgression, apparently took place within the group under study.