Performance Evaluation of the Sysmex XN , XR, and DI ‐60 Body Fluid Applications for Leukocyte Differentiation

INTRODUCTION: In response to the growing demand for automation in body fluid (BF) analysis, we evaluated the research four-part white blood cell (WBC) differentiation provided by the Sysmex XN and XR hematology analyzers (BF mode), as well as the Sysmex digital cell imaging analyzer DI-60 (CellaVision) BF application for microscopic analysis. METHODS: Cerebrospinal, pleural, peritoneal, and synovial fluid samples were analyzed. The four-part WBC differentiation (neutrophil, lymphocyte, monocyte, and eosinophil count (%)) provided by the XN- and XR-BF mode and the DI-60 BF application was compared to manual microscopic examination of May-Grünwald Giemsa stained cytocentrifuge slides. Additionally, the DI-60 BF application was assessed for its capability to detect malignancies. RESULTS: values of 0.735-0.974). CONCLUSIONS: The four-part WBC differentiation provided by the Sysmex XN- and XR-BF mode and the Sysmex DI-60 BF application are valuable tools for automating and standardizing BF analysis.