肝损伤
化学
荧光
酯酶
病态的
荧光寿命成像显微镜
病理
生物化学
人肝
药品
分子探针
作者
Zhen Wang,Yi-Ran Shu,Ying Luo,Dongmei Xi,Tian‐Bing Ren,Lin Yuan,Xiaopeng Fan
标识
DOI:10.1021/acs.jmedchem.5c03044
摘要
Efficient imaging and differentiation of the severity of drug-induced liver injury (DILI) under diabetic conditions are crucial for preclinical drug safety evaluation. However, due to the absence of robust and distinctive parameters, most of the reported fluorescence probes fail to provide prominent imaging for differentiating severity of DILI during the progression of diabetes. Herein, a novel NIR fluorescent probe (WE) with high sensitivity toward esterase activity is developed, underscoring its potential for differentiating the severity of DILI from diabetes-induced hepatotoxicity. Inspiringly, probe WE can clearly differentiate between acetaminophen-induced DILI and diabetes-aggravated DILI by a significantly reduced fluorescence signal, resulting from ROS-mediated esterase inactivation and decreased esterase levels associated with synergistic liver dysfunction. This study not only extends the fluorescence probe’s capability for differential imaging from the liver injury microenvironment to chronic hepatopathy but also provides an additional dimension for differentially elucidating the pathological progression of disease-associated DILI.
科研通智能强力驱动
Strongly Powered by AbleSci AI