明胶
伤口愈合
壳聚糖
自愈水凝胶
京尼平
体内
生物相容性
金黄色葡萄球菌
海水
复合数
化学
生物医学工程
材料科学
外科
医学
复合材料
细菌
高分子化学
生物化学
有机化学
生物技术
地质学
海洋学
生物
遗传学
作者
Qi Fang,Zexin Yao,Longbao Feng,Tian Liu,Shikun Wei,Pengcheng Xu,Rui Guo,Biao Cheng,Xinlu Wang
标识
DOI:10.1016/j.ijbiomac.2020.05.126
摘要
Skin damaged during sea battles is vulnerable to seawater immersion and bacterial infection. Scaffolds with effective biological function are highly desired for treatment of naval combat wound injuries. Herein, we prepared composite scaffolds of CS/GEL/GMs-CIP. The chitosan (CS) and gelatin (GEL) were cross-linked by genipin as matrix, and then gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP) were add. From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility. Furthermore, CS/GEL/GMs-CIP composite scaffold had excellent biocompatibility. Antibacterial experiments confirmed that CS/GEL/GMs-CIP had a significant inhibitory effect on E. coli, S. aureus and P. aeruginosa. The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition. Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated. In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
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