基因
大肠杆菌
克隆(编程)
酶
梭菌
细菌
生物化学
生物
分子克隆
蛋氨酸
分子质量
蛋白质亚单位
分子生物学
化学
基因表达
氨基酸
遗传学
程序设计语言
计算机科学
作者
Vitalia V. Kulikova,Natalya V. Anufrieva,Mikhail I. Kotlov,Elena A. Morozova,V. P. Koval,Yury Belyi,Svetlana V. Revtovich,Tatyana V. Demidkina
标识
DOI:10.1016/j.pep.2020.105810
摘要
The gene NT01CX_1210 of pathogenic bacterium Clostridium novyi annotated as encoding O-acetylhomoserine sulfhydrylase was cloned and expressed in Escherichia coli. The gene product having O-acetylhomoserine sulfhydrylase activity was purified to homogeneity. The protein showed molecular mass of approximately 184 kDa for the native form and 46 kDa for the subunit. The enzyme catalyzes the γ-substitution reaction of O-acetylhomoserine with maximum activity at pH 7.5. Analysis of C. novyi genome allowed us to suggest that there is only one way for the synthesis of l-methionine in the bacterium. The data obtained may provide the basis for further study of the role of OAHS in Clostridium bacteria and an ascertainment of its mechanism.
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