化学
生物分子
分析物
生物传感器
组合化学
生物素
碱性磷酸酶
酶
硝基还原酶
点击化学
试剂
小分子
分子探针
磷酸酶
纳米技术
生物化学
色谱法
有机化学
材料科学
DNA
作者
Chia‐Lin Wu,Chen‐Yo Fan,Chun‐Cheng Lin,Kui‐Thong Tan
标识
DOI:10.1002/jccs.202000200
摘要
Abstract Array‐based analytical platforms have a distinct advantage of being able to detect analytes rapidly and simultaneously with the use of very small quantities of reagents and samples. However, the analyses of enzyme activities using this technique remain challenging, as the heterogeneous interface between the enzyme in the solution and the probe on the array surface impede the efficient binding and the subsequent enzymatic reaction. In this paper, we showed that the combination of affinity‐switchable biotin (ASB) probes and a novel click‐chemistry based immobilization method can overcome this heterogeneity problem. Three ASB probes were synthesized for the detection of alkaline phosphatase, nitroreductase, and the fluoride ion. This approach can be applied to determine the relative levels of phosphatase in different cell lines. We believe that the new strategy holds great potential for the effective sensing of a wide range of biomolecules in the future.
科研通智能强力驱动
Strongly Powered by AbleSci AI