体内
体外
癌症研究
质粒
细胞生长
基因沉默
生物
RNA干扰
细胞培养
小干扰RNA
生长抑制
宫颈癌
宫颈癌
分子生物学
转染
癌症
化学
基因
核糖核酸
生物化学
遗传学
作者
Xin Li,Yang Li,Jiadi Hu,Bo Wang,Lijing Zhao,Kun Ji,Baofeng Guo,Di Yin,Yanwei Du,Dennis J. Kopecko,Dhananjaya V. Kalvakolanu,Xin Zhao,Dihua Xu,Ling Zhang
出处
期刊:Cancer Letters
[Elsevier]
日期:2013-07-01
卷期号:335 (1): 242-250
被引量:25
标识
DOI:10.1016/j.canlet.2013.02.034
摘要
The E6 protein of the oncogenic HPV-16 functions by interfering with the normal cell cycle control mechanisms, particularly those controlled by p53. In this study, we developed a dual expression plasmid that coexpressed-E6-specific siRNA and wild type p53, and to evaluate its effects on cervical cancer growth. We found that simultaneous expression of pSi-E6-P53 caused a robust suppression of tumor growth when compared to the controls either E6-specific siRNA or p53 alone. In conclusion, our findings demonstrate that a combined strategy of co-expressed E6-specific siRNA and p53 synergistically and more effectively suppressed cervical tumor growth when compared with single treatment.
科研通智能强力驱动
Strongly Powered by AbleSci AI