糖
梨
代谢途径
蔗糖
蛋白质组
生物
蛋白质组学
生物化学
植物
新陈代谢
基因
作者
Stefan Reuscher,Yoichiro Fukao,Reina Morimoto,Shungo Otagaki,Akira Oikawa,Kanji Isuzugawa,Katsuhiro Shiratake
出处
期刊:Plant and Cell Physiology
[Oxford University Press]
日期:2016-01-10
卷期号:57 (3): 505-518
被引量:24
摘要
During their 6 month development, pear ( Pyrus communis ) fruits undergo drastic changes in their morphology and their chemical composition. To gain a better understanding of the metabolic pathways and transport processes active during fruit development, we performed a time-course analysis using mass spectrometry (MS)-based protein identification and quantification of fruit flesh tissues. After pre-fractionation of the samples, 2,841 proteins were identified. A principal component analysis (PCA) separated the samples from seven developmental stages into three distinct clusters representing the early, mid and late developmental phase. Over-representation analysis of proteins characteristic of each developmental phase revealed both expected and novel biological processes relevant at each phase. A high abundance of aquaporins was detected in samples from fruits in the cell expansion stage. We were able quantitatively to reconstruct basic metabolic pathways such as the tricarboxylic acid (TCA) cycle, which indicates sufficient coverage to reconstruct other metabolic pathways. Most of the enzymes that presumably contribute to sugar accumulation in pear fruits could be identified. Our data indicate that invertases do not play a major role in the sugar conversions in developing pear fruits. Rather, sucrose might be broken down by sucrose synthases. Further focusing on sugar transporters, we identified several putative sugar transporters from diverse families which showed developmental regulation. In conclusion, our data set comprehensively describes the proteome of developing pear fruits and provides novel insights about sugar accumulation as well as candidate genes for key reactions and transport steps.
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