细粒棘球绦虫
生物
多房棘球绦虫
绦虫
聚合酶链反应
粪便
包虫病
微生物学
蠕虫
病毒学
免疫学
基因
遗传学
动物
作者
Ibrahim Abbasi,A. I. Branzburg,Maiza Campos Ponce,Sami K Abdel Hafez,Francis Raoul,Philip S. Craig,Joseph Hamburger
出处
期刊:American Journal of Tropical Medicine and Hygiene
[American Society of Tropical Medicine and Hygiene]
日期:2003-09-01
卷期号:69 (3): 324-330
被引量:121
标识
DOI:10.4269/ajtmh.2003.69.324
摘要
Diagnosis of Echinococcus granulosus infection in dogs by detecting adult worms recovered post mortem or purged from the intestines after treatment with arecoline is not suitable for mass screening. Large-scale diagnosis by detection of copro-antigens is useful but only with relatively high intensity infections, and only by genus. To provide a more sensitive and specific diagnosis, a polymerase chain reaction (PCR) assay was developed, that amplified a target repeated sequence (EgG1 Hae III) newly identified in the genome of the common sheep strain of E. granulosus. This repeated sequence consists of approximately 6,900 copies, arranged in tandem, in groups of 2-6 repeats. The corresponding primers used in the PCR easily detected a single egg with no cross-amplification of DNA from closely related cestodes, including E. multilocularis and Taenia spp. Fecal samples from naturally infected dogs, with 2-10,000 E. granulosus worms at necropsy, were all PCR positive, while E. multilocularis or Taenia spp. positive controls as well as non-endemic controls were all PCR negative. This copro-PCR assay was demonstrated to be 100% specific and also detected all necropsy-positive E. granulosus-infected dogs. It is suggested that this copro-PCR assay has the potential for pre-mortem diagnosis of E. granulosus infection even in areas where E. granulosus and E. multilocularis are co-endemic.
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