三萜类
生物
生物合成
植物
酶
生物化学
化学
立体化学
作者
Karel Miettinen,Jacob Pollier,Dieter Buyst,Philipp Arendt,René Csük,Sven Sommerwerk,Tessa Moses,Jan Mertens,Prashant Sonawane,Laurens Pauwels,Asaph Aharoni,José Martins,David R. Nelson,Alain Goossens
摘要
Triterpenoids are widespread bioactive plant defence compounds with potential use as pharmaceuticals, pesticides and other high-value products. Enzymes belonging to the cytochrome P450 family have an essential role in creating the immense structural diversity of triterpenoids across the plant kingdom. However, for many triterpenoid oxidation reactions, the corresponding enzyme remains unknown. Here we characterize CYP716 enzymes from different medicinal plant species by heterologous expression in engineered yeasts and report ten hitherto unreported triterpenoid oxidation activities, including a cyclization reaction, leading to a triterpenoid lactone. Kingdom-wide phylogenetic analysis of over 400 CYP716s from over 200 plant species reveals details of their evolution and suggests that in eudicots the CYP716s evolved specifically towards triterpenoid biosynthesis. Our findings underscore the great potential of CYP716s as a source for generating triterpenoid structural diversity and expand the toolbox available for synthetic biology programmes for sustainable production of bioactive plant triterpenoids.
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