依那普利
生物等效性
依那普利
代谢物
生物利用度
药理学
蛋白质沉淀
活性代谢物
化学
生物分析
药代动力学
色谱法
医学
血管紧张素转换酶
内科学
生物化学
血压
作者
Dhiman Halder,Shubhasis Dan,Murari Mohun Pal,Easha Biswas,Nilendra Chatterjee,Pradipta Sarkar,Umesh Chandra Halder,Tapan Kumar Pal
出处
期刊:Future Science OA
[Future Science Ltd]
日期:2017-03-01
卷期号:3 (1): FSO165-FSO165
被引量:10
标识
DOI:10.4155/fsoa-2016-0071
摘要
Background: Enalapril (EPL) is an angiotensin-converting enzyme inhibitor for the treatment of hypertension and chronic heart failure. Enalaprilat (EPLT) is an active metabolite that contributes to the overall activity of EPL. Aim: To quantitate EPL along with its metabolite EPLT using LC–MS/MS, a bioanalytical method was developed and validated with tolbutamide in human plasma using a protein precipitation technique. Results: The sensitive and selective method has an LLOQ of 1 ng/ml with a linearity range of 1–500 ng/ml for both EPL and EPLT using 300 µl of plasma without any matrix effect. Conclusion: Linearity, specificity, accuracy, precision and stability, as well as its application to the analysis of plasma samples after oral administration of 20 mg of EPL maleate in healthy volunteers demonstrate applicability to bioavailability/bioequivalence studies.
科研通智能强力驱动
Strongly Powered by AbleSci AI