Ultrasensitive Pd nano catalyst as peroxidase mimetics for colorimetric sensing and evaluation of antioxidants and total polyphenols in beverages and fruit juices

Herein, we developed a new Pd NP from the aq extract of Elsholtzia blanda Benth. flower that showed efficient peroxidase mimetic activity. The catalytic mechanism was confirmed through colorimetric analysis. The optimizations of temperature, concentration, PH and time were done to find out the best procedure to implement the intrinsic catalytic activity in practical applications. Michaelis-Menten constants were evaluated for both TMB and H2O2 substrate to investigate the affinity of Pd NP towards them. Km was observed to be 42.35 mM for H2O2 and 0.0076 mM for TMB. Antioxidants were sensed using the peroxidase mimetic property up to nanomolar levels with a LOD = 0.78 nM for Gallic acid 0.85 nM for Tannic acid. The method was further implemented in comparing the radical scavenging power of different phenolic compounds. Smart-phone based analysis was done for observing the change in colour which could further be utilized as an analytical tool for study the antioxidant activity. R-Square values of 0.97 and 0.96 for detection of gallic acid and tannic acid respectively suggest good linearity of the plot. Lastly, the system was utilized in the evaluation of total antioxidant capacity (TAC) and total phenolic content (TPC) in commercially available juices and beverages.