Bio-studies of scaffolds based on chitosan/tannic acid cross-linked by glyoxal

Scaffolds based on chitosan/tannic acid cross-linked by glyoxal were obtained by the freeze-frying method. Bio-studies were carried out to consider the safety of material used in medical applications. Thereby, the blood and PDLSC cell compatibility studies were carried out. The results showed that glyoxal is safe cross-linker for chitosan/tannic acid mixtures. Cross-linked scaffolds were nonhemolytic. Moreover, cells cultured on the scaffolds with glyoxal showed higher metabolic activity for 80CTS/20TA composition than others. The current findings allow for the medical application of the proposed materials as wound dressings.