纳米探针
费斯特共振能量转移
化学
拉曼光谱
纳米技术
荧光
过氧化氢
生物物理学
组合化学
生物化学
材料科学
纳米颗粒
生物
量子力学
光学
物理
作者
Qiaoyan Jiang,Ximin Cui,Yang Sun,Zhengsheng Mao,Jie Wang,Feng Chen,Jianfang Wang,Yue Cao
标识
DOI:10.1016/j.bios.2021.113539
摘要
Caspase-3 and hydrogen peroxide (H2O2) are closely associated with numerous diseases, both of them are vital in different physiological and pathological conditions. They are closely related and also can act independently. The selective and accurate determination of caspase-3 and H2O2 simultaneously to determine their state of being in different situations is of great significance for further study of their molecular mechanisms and the elucidation of their biological functions. In our latest research, a AuNPL-crown nanoprobe was obtained by attaching (4-aminosulfonylphenyl) boronic acid (4-APBA) and peptide-FITC (NH2-Asp-Glu-Val-Asp (DEVD)-FITC) to gold nanoplates (AuNPLs). The fabricated AuNPL-crown nanoprobe was used for dual-channel and real-time tracking of the dynamic changes in caspase-3 and H2O2 based on fluorescence resonance energy transfer (FRET)/surface-enhanced Raman spectroscopy (SERS) technology. The AuNPL-crown nanoprobe not only provides synergy but can also achieve noninterference, making the results more reliable and repeatable. This study simultaneously traced the dynamic changes of caspase-3 and H2O2 on a single probe, which provides a potential new platform for the analysis of caspase-3 and H2O2 in the biological environment with high accuracy, sensitivity, convenience, and efficiency. In summary, we develop a new strategy for the simultaneous detection of different substances on a single probe.
科研通智能强力驱动
Strongly Powered by AbleSci AI