生物
神经嵴
干细胞
细胞生物学
胚胎干细胞
神经外胚层
诱导多能干细胞
多能干细胞
CD90型
成体干细胞
定向微分
中胚层
祖细胞
川地34
遗传学
胚胎
基因
作者
Qian Zhu,Mingming Li,Chuan Yan,Qingming Lu,Shichao Wei,Rong Gao,Mengfei Yu,Yu Zou,Gopu Sriram,Huei Jinn Tong,Walter Hunziker,Chaminda Jayampath Seneviratne,Zhiyuan Gong,Bjørn R. Olsen,Tong Cao
标识
DOI:10.1002/biot.201700067
摘要
Neural crest stem cells (NCSCs) are a transient and multipotent cell population giving rise to various cell types with clinical importance. Isolation of human NCSCs is extremely challenging that limits our knowledge about neural crest development and application. Here, a defined protocol to efficiently direct human embryonic stem cells (hESCs) to NCSCs and multiple neural crest lineages is presented. A unique combination of small molecule inhibitors and growth factors is employed to generate NCSCs from hESCs through a neuroectoderm stage. The self‐renewal and multipotent capacities of hESC‐derived NCSCs are assessed subsequently. In the feeder‐free system, hESC‐derived NCSCs (P75 + /HNK1 + /AP2α + /PAX6 − ) in high purity are efficiently generated following neuroectodermal restriction. They can be propagated and differentiated toward multiple neural crest lineages in vitro, such as functional peripheral neurons (β‐tubulin III + /peripherin + ), mesenchymal stem cells (CD73 + CD90 + CD105 + ), and corneal keratocytes (keratocan + ). The in vivo developmental potential of hESC‐derived NCSCs is confirmed using zebrafish embryos. This report is the first demonstration of efficient differentiation of hESCs into corneal keratocytes as a monolayer in a feeder‐free system. Considering the high efficacy of NCSC generation, this new method will be a useful tool for future clinical organ repair and regeneration, such as peripheral nerve regeneration and corneal repair.
科研通智能强力驱动
Strongly Powered by AbleSci AI