赫拉
体外
体内
细胞凋亡
多糖
细胞毒性
化学
硫酸化
细胞培养
分子生物学
细胞生长
生物化学
细胞生物学
生物
遗传学
生物技术
作者
Junlong Wang,Aijuan Bao,Qi Wang,Hongyun Guo,Yongdong Zhang,Junyu Liang,Weibao Kong,Jian Yao,Ji Zhang
标识
DOI:10.1016/j.ijbiomac.2017.09.018
摘要
In this study, a sulfated Artemisia sphaerocephala polysaccharide (ASPs) was prepared and its antitumor activity was evaluated in tumor cells and Hepatoma 22 (H22) tumor-bearing mice. In vitro experiments, ASPs significantly inhibited the growth of HepG2 and Hela cells with the IC50 values of 172.03 and 161.42μg/mL, respectively. Moreover, no direct cytotoxicity against mouse fibroblast L929 normal cells was observed in vitro. After oral administration for 12days, the tumor growth was significantly suppressed by ASPs at the doses of 200mg/kg (inhibition rate of 60.85%). Results of tumor histological morphology and cell cycle analysis showed that ASPs could arrest H22 cells at S phase and promote cell apoptosis. Additionally, immunohistochemical analysis demonstrated that ASPs caused the down-regulation of mutant p53 protein expression in a dose-dependent manner. Therefore, these findings proposed new insight into antitumor properties of sulfated polysaccharide as a promising agent in cancer treatment.
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