纳米器件
DNA
DNA纳米技术
DNA折纸
基质(水族馆)
劈开
纳米技术
生物物理学
酶
化学
材料科学
生物化学
生物
生态学
作者
Guido Grossi,Mette D. E. Jepsen,Jørgen Kjems,Ellen Juul Andersen
标识
DOI:10.1038/s41467-017-01072-8
摘要
Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme-substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme-substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme-substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.
科研通智能强力驱动
Strongly Powered by AbleSci AI