Five compounds were isolated from alcohol extract of Vaccaria segetalis (Neck) Garcke. On the basis of chemical and spectroscopic analysis, they were identified as: Vaccaroside B(1)、 C(2)、 D(3), 6-N-methyl adenosine(4) and N, N-dimethyl-L-trptophan(5). Among them, compounds 4 and 5 were isolated from Vaccaria segetalis (Neck) Garcke for the first time.