Advances in the Modification of Enzymatic Properties Based on Protein Engineering Strategies

Enzymes are environmentally friendly biocatalysts that play a crucial role in catalyzing biochemical reactions. However, the catalytic performance of natural enzymes often cannot fully meet the demands of industrial production. Rapidly developing AI tool-assisted protein engineering modification strategies can be used to significantly enhance the catalytic properties of enzymes. The review highlights the functional features of different protein language models and their representative tools. In addition, the system reviews various software tools used for analyzing protein catalytic performance evaluation indicators and provides a detailed comparison of the success rates and application permissions of different tools. Finally, the challenges and future directions of AI techniques in protein engineering are emphasized to strengthen the role of rational computations in protein design and personalization. This review provides a comprehensive perspective for researchers to promote further development in the field of protein engineering.