天冬氨酸
产量(工程)
大肠杆菌
色谱法
化学
铵
聚丙烯酰胺
氨基酸
生物化学
材料科学
有机化学
高分子化学
基因
冶金
作者
Tetsuya Tosa,Tadashi Satô,Takeo Mori,Ichiro Chibata
出处
期刊:Applied microbiology
[American Society for Microbiology]
日期:1974-05-01
卷期号:27 (5): 886-889
被引量:23
标识
DOI:10.1128/am.27.5.886-889.1974
摘要
By using a column packed with immobilized Escherichia coli cells entrapped in a polyacrylamide gel lattice, conditions for continuous production of L-aspartic acid from ammonium fumarate were investigated. When a solution of 1 M ammonium fumarate (pH 8.5) containing 1 mM Mg 2+ was passed through the immobilized cell column at a flow rate of space velocity (SV) = 0.8 at 37 C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in good yield. The immobilized cell column was very stable.
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