生物分子
共价键
化学
部分
组合化学
聚合物
表面改性
叠氮化物
生物活性
分子
聚乙二醇化
点击化学
生物界面
有机化学
纳米技术
材料科学
体外
生物化学
聚乙二醇
物理化学
作者
Anne-Sophie Mertgen,Anne Géraldine Guex,Samuele Tosatti,Giuseppino Fortunato,René M. Rossi,Markus Rottmar,Katharina Maniura‐Weber,Stefan Zürcher
标识
DOI:10.1016/j.apsusc.2022.152525
摘要
Current biointerfaces aiming to steer specific biological responses frequently lack either stability due to purely electrostatic interactions, bioactivity due to unspecific conjugation chemistries, specificity due to uncontrolled biological interactions such as fouling, or cytocompatibility due to harsh and toxic coating procedures. Here, we report a versatile surface modification platform for covalent tethering of selected biomolecules. New in this approach is the particular combination of modular binding blocks as graft co-polymer. Grafted to the backbone of PAcrAmTM multiple functionalities are strategically combined: covalent (silane) and non-covalent (lysine) surface binding groups for stability and self-assembly in mild buffered solution, PEG-azide chains for low fouling properties, and specific, controlled, covalent, linking of biologically active molecules. This modular strategy overcomes the previously mentioned limitations, for instance regarding bioactivity of the biological moiety due to highly specific strain-promoted azide-alkyne cycloaddition. The successful grafting of the copolymer was confirmed by 1H NMR. The immobilization of RGD peptides was characterized by combining surface analytical techniques, such as ToF-SIMS and ellipsometry, allowing quantification of immobilized molecules over an extensive range of concentrations (0.008–1.95 pmol·cm−2). The bioactivity over this range of concentrations was confirmed by in vitro cell studies, presenting a differential endothelial cell attachment and spreading. The modified substrates enabled the formation of an interconnected monolayer of endothelial cells. Furthermore, the modular platform allowed the co-immobilization of two bioactive functional groups, RGD and biotin, on the same surface, which could be exploited for the further development of controlled multi-functional biointerfaces for diverse biological applications in the future.
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