啶虫脒
适体
检出限
分析物
化学
选择性
灵敏度(控制系统)
再现性
材料科学
色谱法
纳米技术
分析化学(期刊)
杀虫剂
生物
分子生物学
生物化学
电子工程
益达胺
农学
工程类
催化作用
作者
Jie Zhou,Dan Wang,Haifeng Yang,Feng Wang
标识
DOI:10.1016/j.saa.2021.120801
摘要
Acetamiprid poses a serious threat to human beings. In this work, the highly transparent fluorescent quantitative PCR sealing membranes (PCR-M) developed in our previous work which had attractive characteristics such as sensitivity, stability and reproducibility, and its capability of extracting analytes quickly, was used to detect acetamiprid. The detection limit of 1 nmol/L was obtained by direct detection of CN group in acetamiprid. In order to improve the selectivity and sensitivity, acetamiprid aptamer was introduced. After the interaction of aptamer with acetamiprid the SERS signal of adenine at 730 cm-1 increased. The detection limit of indirect detection was 10-8 mol/L. Aptamer advanced Ag-PCR-M-based Surface enhanced Raman spectroscopy (SERS) method for indirect SERS detection which significantly improves the detection sensitivity and greatly increases the range of signal response value. This will be conducive to the detection of actual samples and expand the practical application.
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