根癌农杆菌
转化(遗传学)
生物
农杆菌
基因组
基因
植物细胞
遗传学
DNA
植物科学
计算生物学
生物技术
植物
作者
Hau-Hsuan Hwang,Manda Yu,Erh‐Min Lai
出处
期刊:The Arabidopsis Book
[BioOne- American Society of Plant Biologists (Arabidopsis)]
日期:2017-01-01
卷期号:15: e0186-e0186
被引量:156
摘要
Plant genetic transformation heavily relies on the bacterial pathogen Agrobacterium tumefaciens as a powerful tool to deliver genes of interest into a host plant. Inside the plant nucleus, the transferred DNA is capable of integrating into the plant genome for inheritance to the next generation (i.e. stable transformation). Alternatively, the foreign DNA can transiently remain in the nucleus without integrating into the genome but still be transcribed to produce desirable gene products (i.e. transient transformation). From the discovery of A. tumefaciens to its wide application in plant biotechnology, numerous aspects of the interaction between A. tumefaciens and plants have been elucidated. This article aims to provide a comprehensive review of the biology and the applications of Agrobacterium-mediated plant transformation, which may be useful for both microbiologists and plant biologists who desire a better understanding of plant transformation, protein expression in plants, and plant-microbe interaction.
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