化学
DNA
小RNA
四面体
细胞内
荧光
纳米颗粒
荧光寿命成像显微镜
分子生物学
纳米技术
催化作用
生物物理学
计算生物学
生物化学
基因
结晶学
生物
物理
材料科学
量子力学
作者
Liuting Mo,Mingxiu Mo,Danlian Liang,Chan Yang,Weiying Lin
出处
期刊:Talanta
[Elsevier]
日期:2023-12-01
卷期号:265: 124871-124871
被引量:2
标识
DOI:10.1016/j.talanta.2023.124871
摘要
Improving the accuracy, sensitivity and speed of intracellular miRNA imaging is essential for early diagnosis of cancer. To achieve this goal, we herein present a strategy for imaging two distinct miRNAs by DNA tetrahedron-based catalytic hairpin assembly (DCHA). Two nanoprobes, DTH-13 and DTH-24, were prepared by one-pot synthesis. The resultant structures were DNA tetrahedrons functionalized with two sets of CHA hairpins, which respectively responded to miR-21 and miR-155. Using these structured DNA nanoparticles as the carriers, the probes could easily enter living cells. The presence of miR-21 or miR-155 could trigger CHA between DTH-13 and DTH-24, leading to independent fluorescence signals of FAM and Cy3. In this system, the sensitivity and kinetics were significantly enhanced owing to the strategy of DCHA. The sensing performance of our method was thoroughly investigated in buffers, fetal bovine serum (FBS) solutions, living cells, and clinical tissue samples. The results validated the potential of DTH nanoprobes as a diagnostic tool for early stages of cancer.
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