Hydrochar reduces oxytetracycline in soil and Chinese cabbage by altering soil properties, shifting microbial community structure and promoting microbial metabolism

The efficient remediation of antibiotic-contaminated soil is critical for agroecosystem and human health. Using the cost-effective and feedstock-independent hydrochar with rich oxygen-containing functional groups as a soil remediation material has become a hot concern nowadays. However, the feasibility and effectiveness of hydrochar amendment in antibiotic-contaminated soil still remain unknown. Therefore, this study investigated the remediation effect and potential mechanisms of different hydrochars from cow manure (H-CM), corn stalk (H-CS) and Myriophyllum aquaticum (H-MA) at two levels (0.5% and 1.0%) in oxytetracycline (OTC)-contaminated soil using a pot experiment. Results showed that compared with CK, OTC content in the soils amended with H-CM and H-MA was decreased by 14.02-15.43% and 9.23-24.98%, respectively, whereas it was increased by 37.03-42.64% in the soils amended with H-CS. Additionally, all hydrochar amendments effectively reduced the OTC uptake in root and shoot of Chinese cabbage by 10.41-57.99% and 31.92-65.99%, respectively. The response of soil microbial community to hydrochar amendment heavily depended on feedstock type rather than hydrochar level. The soil microbial metabolism (e.g., carbohydrate metabolism, amino acid metabolism) was enhanced by hydrochar amendment. The redundancy analysis suggested that TCA cycle was positively related to the abundances of OTC-degrading bacteria (Proteobacteria, Arthrobacter and Sphingomonas) in all hydrochar-amended soils. The hydrochar amendment accelerated the soil OTC removal and reduced plant uptake in soil-Chinese cabbage system by altering soil properties, enhancing OTC-degrading bacteria and promoting microbial metabolism. These findings demonstrated that the cost-effective and sustainable hydrochar was a promising remediation material for antibiotic-contaminated soil.