孢子萌发
孢子
生物
黄曲霉
转录组
细胞生物学
柠檬酸循环
生物化学
超氧化物歧化酶
线粒体
琥珀酸脱氢酶
微生物学
新陈代谢
氧化应激
基因表达
基因
作者
Yaxin Wang,Mingming Yu,Yanli Xie,Weibin Ma,Shanwen Sun,Qian Li,Yuhui Yang,Xiao Li,Jia Hao,Renyong Zhao
出处
期刊:Toxicon
[Elsevier]
日期:2024-02-01
卷期号:239: 107615-107615
标识
DOI:10.1016/j.toxicon.2024.107615
摘要
Dielectric barrier discharge plasma (DBDP) displays strong against fungal spores, while its precise mechanism of spore inactivation remains inadequately understood. In this study, we applied morphological, in vivo and in vitro experiments, transcriptomics, and physicochemical detection to unveil the potential molecular pathways underlying the inactivation of Aspergillus flavus spores by DBDP. Our findings suggested that mycelium growth was inhibited as observed by SEM after 30 s treatment at 70 kV, meanwhile spore germination ceased and clustering occurred. It led to the release of cellular contents and subsequent spore demise by disrupting the integrity of spore membrane. Additionally, based on the transcriptomic data, we hypothesized that the induction of spore inactivation by DBDP might be associated with downregulation of genes related to cell membranes, organelles (mitochondria), oxidative phosphorylation, and the tricarboxylic acid cycle. Subsequently, we validated our transcriptomic findings by measuring the levels of relevant enzymes in metabolic pathways, such as superoxide dismutase, acetyl-CoA, total dehydrogenase, and ATP. These physicochemical indicators revealed that DBDP treatment resulted in mitochondrial dysfunction, redox imbalance, and inhibited energy metabolism pathways. These findings were consistent with the transcriptomic results. Hence, we concluded that DBDP accelerated spore rupture and death via ROS-mediated mitochondrial dysfunction, which does not depend on cell membranes.
科研通智能强力驱动
Strongly Powered by AbleSci AI