静电纺丝
生物材料
纳米复合材料
材料科学
生物相容性
接触角
膜
化学工程
热重分析
表面改性
纳米纤维
傅里叶变换红外光谱
MTT法
核化学
化学
纳米技术
聚合物
细胞生长
复合材料
生物化学
冶金
工程类
作者
Balaganesh Danagody,Neeraja Bose,Kalaivizhi Rajappan,Anwar Iqbal,Ganesh Munuswami Ramanujam,Aswathy Karanath Anilkumar
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2023-12-11
标识
DOI:10.1021/acsbiomaterials.3c00892
摘要
Developing biomaterial scaffolds using tissue engineering with physical and chemical surface modification processes can improve the bioactivity and biocompatibility of the materials. The appropriate substrate and site for cell attachment are crucial in cell behavior and biological activities. Therefore, the study aims to develop a conventional electrospun nanofibrous biomaterial using reproducible surface topography, which offers beneficial effects on the cell activities of bone cells. The bioactive MgO/gC3N4 was incorporated on PAN/PEG and fabricated into a nanofibrous membrane using electrospinning. The nanocomposite uniformly distributed on the PAN/PEG nanofiber helps to increase the number of induced pores and reduce the hydrophobicity of PAN. The physiochemical characterization of prepared nanoparticles and nanofibers was carried out using FTIR, X-ray diffraction (XRD), thermogravimetry analysis (TGA), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements. SEM and TEM analyses examined the nanofibrous morphology and the structure of MgO/gC3N4. In vitro studies such as on ALP activity demonstrated the membrane’s ability to regenerate new bone and healing capacity. Furthermore, alizarin red staining showed the increasing ability of the cell–cell interaction and calcium content for tissue regeneration. The cytotoxicity of the prepared membrane was about 97.09% of live THP-1 cells on the surface of the MgO/gC3N4@PAN/PEG membrane evaluated using MTT dye staining. The soil burial degradation analysis exhibited that the maximum degradation occurs on the 45th day because of microbial activity. In vitro PBS degradation was observed on the 15th day after the bulk hydrolysis mechanism. Hence, on the basis of the study outcomes, we affirm that the MgO/gC3N4@PAN/PEG nanofibrous membrane can act as a potential bone regenerative substrate.
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