The Prototypical Oligopeptide Transporter YdgR From E. coli Exhibits a Strict Preference for β‐Ala‐Lys(AMCA)

ABSTRACT Fluorescent probes are widely used in cellular imaging and disease diagnosis. Acting as substitute carriers, fluorescent probes can also be used to help transport drugs within cells. In this study, commonly used fluorophores, TAMRA (5‐carboxytetramethylrhodamine), PBA (1‐pyrenebutyric acid), NBD (nitrobenzoxadiazole), OG (Oregon Green), and CF (5‐carboxyfluorescein) were conjugated with the dipeptide β‐Ala‐Lys, the peptide moiety of the well‐established peptide transporter substrate β‐Ala‐Lys(AMCA) (AMCA: 7‐amino‐4‐methyl‐coumarin‐3‐acetic acid) by modifying it with respect to side‐chain length and functional end groups. The analogs were tested for transport through or inhibition of YdgR, a prototypical peptide transporter from E. coli and apparently homologous to the human PEPT1. Strikingly, none of the dipeptide‐fluorophore conjugates nor minor modifications in the reporter substrate were tolerated by YdgR, indicating discrepancies to PEPT1. These findings underscore intricate substrate recognition mechanisms governing substrate recognition by YdgR.