磷酸化
拟南芥
细胞生物学
化学
信号转导
硝酸盐
平衡
泛素
生物化学
生物
基因
突变体
有机化学
作者
Xiaohan Wang,Yongqiang Liu,Weiwei Li,Xiaojun Ma,Wei Wang,Zhimin Jiang,Yiqin Wang,Legong Li,Bin Hu,Chengcai Chu
标识
DOI:10.1002/advs.202507919
摘要
Nitrate, a crucial nutrient and signaling molecule, is extensively studied across plants. While the NRT1.1-NLP-centered pathway dominates nitrate signaling in Arabidopsis and rice, however, whether there is functional interaction or co-regulation between the primary nitrate response (PNR) and long-term nitrogen utilization remains unclear. Here, a novel nitrate signaling pathway is identified in rice that works alongside the established ubiquitination-mediated OsNRT1.1B-OsSPX4-OsNLP3 cascade. It is demonstrated that OsCNGC14, OsCNGC16, and OsNRT1.1B form a plasma membrane-localized complex in root tips, mediating nitrate-triggered Ca2⁺ influx. The absence of either OsCNGC14 or OsCNGC16 abolished Ca2⁺ signaling and suppressed PNR. The OsNRT1.1B-OsCNGC14/16 complex activates Ca2⁺-dependent phosphorylation of OsNLP3 at Ser193, which accelerates its nuclear translocation and transcriptional activation of nitrate-responsive genes. This phosphorylation enhances both short-term PNR and long-term nitrogenutilization. This findings reveal a dual regulatory network in rice: the Ca2⁺-OsNLP3 pathway rapidly amplifies nitrate signals, while the ubiquitination-mediated OsSPX4 degradation ensures sustained nitrogen homeostasis.
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