Brachymonas wangyanguii sp. nov., isolated from the coal samples in Shanxi Province of China

Two Gram-negative, oxidase-positive, catalase-positive, non-spore-forming, motile, short-rod strains (G13 T and G34) were isolated from in situ coal block samples. Phylogenetic analyses based on 16S rRNA genes and 718 core genes indicated that both strains belonged to the genus Brachymonas , with the closest relative being Brachymonas chironomi AIMA4 T as their closest relative. Average nucleotide identity and digital DNA–DNA hybridization values were 79.4–89.27% and 23.6–37.0% below the species-level thresholds. Further chemotaxonomic analysis revealed that both strains possessed C 16:1 ω 7 c and/or C 16:1 ω 6 c , C 18:1 ω 7 c and C 16:0 as major cellular fatty acids, with Q-8 as the predominant respiratory quinone. The polar lipid profile was dominated by diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phenotypic and phylogenetic evidence support the proposal that strain G13 T represents a novel species of the genus Brachymonas , with the name Brachymonas wangyanguii sp. nov. The type strain is G13 T (=GDMCC 1.4691 T =JCM 37746 T ).