Protective Effect of Fish Gut Hydrolysates from Olive Flounder (Paralichthys olivaceus) Surimi Byproducts Against AAPH-Induced Oxidative Stress in In Vitro and In Vivo Zebrafish Models

Olive flounder is a common surimi industry related fish species in South Korea that generates a considerable amount of solid waste. To maximize the utilization of olive flounder gut (OFG) byproducts, OFG was subjected to enzymatic hydrolysis. Antioxidant activity of the obtained hydrolysates was evaluated to investigate radical scavenging activity (RSA), and in vitro experiments were conducted on 2,2’-azobis(2-amidinopropane) dihydrochloride-stimulated Vero cells. Protamex hydrolysates showed a strong RSA (IC50 value of 94.96 ± 1.60 µg/mL) with a significant reduction in oxidative stress in in vitro and in vivo zebrafish models. OFG byproducts have potential for industrial application as antioxidative agents.