Crystallization of 4-hydroxybenzoyl-CoA reductase and the structure of its electron donor ferredoxin

铁氧还蛋白 化学 氧化还原酶 结晶 还原酶 氧化还原 结晶学 分子置换 电子转移 立体化学 晶体结构 生物化学 有机化学
作者
Mihaela Unciuleac,Matthias Boll,Eberhard Warkentin,Ulrich Ermler
出处
期刊:Acta Crystallographica Section D-biological Crystallography [Wiley]
卷期号:60 (2): 388-391 被引量:17
标识
DOI:10.1107/s0907444903028506
摘要

4-Hydroxybenzoyl-CoA reductase (4-HBCR) is a central enzyme in the metabolism of phenolic compounds in anaerobic bacteria. The enzyme catalyzes the reductive removal of the phenolic hydroxyl group from 4-hydroxybenzoyl-CoA, yielding benzoyl-CoA and water. 4-HBCR belongs to the xanthine oxidase (XO) family of molybdenum enzymes which occur as heterodimers, (αβγ)2. 4-HBCR contains two molybdopterins, four [2Fe–2S] and two [4Fe–4S] clusters and two FADs. A low-potential Allochromatium vinosum-type ferredoxin containing two [4Fe-4S] clusters serves as an in vivo electron donor for 4-HBCR. In this work, the oxygen-sensitive proteins 4-HBCR and the ferredoxin (TaFd) from Thauera aromatica were crystallized under anaerobic conditions. 4-HBCR crystallized with PEG 4000 and MPD as precipitant diffracted to about 1.6 Å resolution and the crystals were highly suitable for X-ray structure analysis. Crystals of TaFd were obtained with (NH4)3PO4 as precipitant and revealed a solvent content of 77%, which is remarkably high for a small soluble protein. The structure of TaFd was solved at 2.9 Å resolution by the molecular-replacement method using the highly related structure of the ferredoxin (CvFd) from A. vinosum as a model. Structural changes between the two ferredoxins around the [4Fe–4S] cluster can be correlated with their different redox potentials.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
joker_k应助科研通管家采纳,获得20
刚刚
英姑应助科研通管家采纳,获得10
刚刚
英俊的铭应助科研通管家采纳,获得10
刚刚
刚刚
科研通AI5应助科研通管家采纳,获得10
刚刚
我是老大应助科研通管家采纳,获得10
1秒前
joker_k应助科研通管家采纳,获得20
1秒前
安阳发布了新的文献求助20
1秒前
行云流水完成签到,获得积分10
1秒前
2秒前
Joker完成签到,获得积分10
3秒前
浔初先生发布了新的文献求助10
3秒前
4秒前
喜悦的飞机完成签到,获得积分10
6秒前
7秒前
8秒前
彭于晏应助nancy采纳,获得30
8秒前
111111完成签到,获得积分10
9秒前
10秒前
10秒前
微笑完成签到,获得积分10
10秒前
zxvcbnm完成签到,获得积分10
12秒前
12秒前
12秒前
12秒前
小蘑菇应助土豆酱采纳,获得10
13秒前
林非鹿完成签到 ,获得积分10
13秒前
香蕉觅云应助香蕉汉堡采纳,获得10
14秒前
新手完成签到 ,获得积分10
15秒前
16秒前
灯灯完成签到,获得积分10
18秒前
云下完成签到 ,获得积分10
18秒前
18秒前
任性的卿完成签到,获得积分10
19秒前
pengyang完成签到 ,获得积分10
19秒前
Hollen发布了新的文献求助10
19秒前
21秒前
oblivious完成签到,获得积分10
22秒前
Chenglong关注了科研通微信公众号
24秒前
灰太狼大王完成签到 ,获得积分10
24秒前
高分求助中
Introduction to Strong Mixing Conditions Volumes 1-3 500
Tip60 complex regulates eggshell formation and oviposition in the white-backed planthopper, providing effective targets for pest control 400
Optical and electric properties of monocrystalline synthetic diamond irradiated by neutrons 320
共融服務學習指南 300
Essentials of Pharmacoeconomics: Health Economics and Outcomes Research 3rd Edition. by Karen Rascati 300
Peking Blues // Liao San 300
Political Ideologies Their Origins and Impact 13 edition 240
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3801165
求助须知:如何正确求助?哪些是违规求助? 3346853
关于积分的说明 10330624
捐赠科研通 3063166
什么是DOI,文献DOI怎么找? 1681445
邀请新用户注册赠送积分活动 807567
科研通“疑难数据库(出版商)”最低求助积分说明 763728