Biosynthetic pathways and detection methods of protein O-GlcNAc glycosylation modification

Protein O-linked N-acetylglucosamine (O-GlcNAc) glycosylation modification is one of the most important contents of protein post translation modification (PTM). Most of the protein O-GlcNAc glycosylation modifications take place at cell nucleus and cytoplasm. During the process of protein O-GlcNAc glycosylation modification, O-linked N-acetylglucosaminyltransferase (OGT) and O-linked N-acetylglucosaminidase (OGA) together maintain the stability of intracellular glycosylation levels. Protein O-GlcNAc glycosylation modification can dynamically regulate the functions of various enzymes in cell signal transduction pathway, and it plays an important regulatory role in many life activities, and it is closely related to the occurrence of various metabolic diseases such as gestational diabetes. The authors intend to review the latest research progress of the biosynthetic pathways and detection methods of protein O-GlcNAc glycosylation. Key words: Glycosylation; Databases, protein; O-linked N-acetylglucosamine; O-linked N-acetylglucosaminyltransferase; O-linked N-acetylglucosaminidase; Diabetes, gestational; Detection methods