肺癌
PI3K/AKT/mTOR通路
癌症研究
癌症
细胞凋亡
蛋白激酶B
细胞生长
FGF21型
生物
医学
成纤维细胞生长因子
病理
内科学
受体
生物化学
作者
Xiaofeng Yu,Ying Li,Guodong Jiang,Jian Fang,Zhaolei You,Guangyuan Shao,Zheng Zhang,Aihong Jiao,Xiaonu Peng
出处
期刊:Life Sciences
[Elsevier]
日期:2020-12-10
卷期号:269: 118875-118875
被引量:35
标识
DOI:10.1016/j.lfs.2020.118875
摘要
Lung cancer is a key contributor to the cancer-related death throughout the world. FGF21 (fibroblast growth factor 21) has been found to regulate various pulmonary diseases, whereas, the role and mechanism of FGF21 in lung cancer remain unclear. The aim of this research was to explore the expression and function of FGF21 in lung cancer. The mRNA and protein expression of FGF21 were analyzed through qRT-PCR and western blot, respectively. Cell proliferation, apoptosis and migration were analyzed by CCK-8 assay, flow cytometry and wound-healing assay, respectively. ROS, SOD, LDH and CK were examined with respective commercially kit. FGF21 level was increased in lung cancer tissue samples and cell lines at both mRNA and protein levels. Overexpressing FGF21 promoted cell growth and migration significantly. It also increased SOD and reduced ROS, LDH and CK contents. By contrast, down-regulated FGF21 presented the opposite effect on lung cancer cells. Furthermore, FGF21 may function as a tumor promotor by activating the SIRT1/PI3K/AKT signaling pathway in lung cancer. This study demonstrated that FGF21 was a tumor promoter in lung cancer development, serving as a feasible therapeutic target in the treatment of lung cancer.
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