生物
代谢组学
转录组
蛋白质组
接种
陈皮
代谢物
病菌
园艺
植物
微生物学
基因
基因表达
生物化学
生物信息学
半翅目
作者
John S. Ramsey,Elizabeth Chin,Juan D. Chavez,Surya Saha,Darya Mischuk,Jaclyn Mahoney,Jared P. Mohr,Faith M. Robison,Elizabeth Mitrovic,Youqiang Xu,Susan R. Strickler,Noe Fernandez,Xuefei Zhong,MaryLou Polek,Kris Godfrey,James J. Giovannoni,Lukas A. Mueller,Carolyn M. Slupsky,James E. Bruce,Michelle Heck
标识
DOI:10.1021/acs.jproteome.9b00802
摘要
Presymptomatic detection of citrus trees infected with Candidatus Liberibacter asiaticus (CLas), the bacterial pathogen associated with Huanglongbing (HLB; citrus greening disease), is critical to controlling the spread of the disease. To test whether infected citrus trees produce systemic signals that may be used for indirect disease detection, lemon (Citrus limon) plants were graft-inoculated with either CLas-infected or control (CLas−) budwood, and leaf samples were longitudinally collected over 46 weeks and analyzed for plant changes associated with CLas infection. RNA, protein, and metabolite samples extracted from leaves were analyzed using RNA-Seq, mass spectrometry, and 1H NMR spectroscopy, respectively. Significant differences in specific transcripts, proteins, and metabolites were observed between CLas-infected and control plants as early as 2 weeks post graft (wpg). The most dramatic differences between the transcriptome and proteome of CLas-infected and control plants were observed at 10 wpg, including coordinated increases in transcripts and proteins of citrus orthologs of known plant defense genes. This integrated approach to quantifying plant molecular changes in leaves of CLas-infected plants supports the development of diagnostic technology for presymptomatic or early disease detection as part of efforts to control the spread of HLB into uninfected citrus groves.
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