生物膜
肠沙门氏菌
毒力
微生物学
伞
血清型
沙门氏菌
生物
突变体
拉伤
菌毛
细菌
基因
生物化学
遗传学
解剖
作者
Muhanad El Hag,Zijian Feng,Yangyang Su,Xiao Wang,Afrah Kamal Yassin,Sujuan Chen,Daxin Peng,Xiufan Liu
标识
DOI:10.1080/03079457.2017.1324198
摘要
Salmonella biofilm formation is important to environmental stress resistance and virulence. However, the roles of the csgA and bcsA genes, which affect curli protein and cellulose production, respectively, in Salmonella enterica serovar Pullorum, are unknown. Here we constructed deletions in the csgA and bcsA genes in S. enterica serovar Pullorum strain S6702 and evaluated several aspects of biofilm formation and virulence. ΔcsgA showed decreased production of curli fimbriae, while ΔbcsA had reduced cellulose production. Both mutants had a reduced ability to form biofilms. ΔcsgA was reduced in adhesion and invasion to HeLa cells and exhibited decreased intracellular proliferation in HD11 macrophages. ΔbcsA exhibited increased proliferation in HD11 cells and replicated better in chicken spleens, as compared to the wild-type strain. ΔcsgA virulence was attenuated in assays involving oral challenge of one-day-old chickens.
科研通智能强力驱动
Strongly Powered by AbleSci AI