A microfluidic skin-on-a-chip enabling in situ construction of full-thickness human skin for modeling inflammatory diseases

formation of a hierarchically organized full-thickness skin model with appropriate epidermal stratification and differentiation marker expression under a sustained dynamic culture. As a proof-of-concept application, a Th2 cytokine-driven inflammatory skin phenotype was established on-chip, recapitulating key molecular features of barrier impairment and inflammation. Pharmacological intervention induced quantifiable, reversible molecular responses, demonstrating the platform's ability to distinguish inflammatory and treatment states in a dynamically maintained skin microenvironment. Collectively, this work presents a practical microfluidic strategy for process-integrated skin tissue construction, offering an experimentally accessible framework for inflammatory skin modeling and drug evaluation in organ-on-a-chip systems.