Kinetic and structural analysis of the group I catalytic ribozyme utilizing fluorescent probes (743.2)

Group I introns are catalytic RNAs capable of performing a range of phospho‐transesterification reactions including self‐splicing and RNA cleavage. This study focuses on the Tetrahymena and Anabaena Group I ribozymes as models. This work describes the application of steady‐state gel based assays utilizing fluorescently labeled substrate or ribozymes to evaluate structure function relationships within the ribozymes. We report the initial results from the application of this technique to a study of variants of the P1 substrate‐binding domain of the Anabaena Group I ribozyme.