脱落酸
硝酸盐
拟南芥
生物化学
拟南芥
激酶
磷酸酶
化学
细胞生物学
生物
酶
基因
突变体
有机化学
作者
Sophie Léran,Kai H. Edel,Marjorie Pervent,Kenji Hashimoto,Claire Corratgé-Faillie,Jan Niklas Offenborn,Pascal Tillard,Alain Gojon,Jörg Kudla,Benoı̂t Lacombe
出处
期刊:Science Signaling
[American Association for the Advancement of Science (AAAS)]
日期:2015-05-05
卷期号:8 (375)
被引量:170
标识
DOI:10.1126/scisignal.aaa4829
摘要
Living organisms sense and respond to changes in nutrient availability to cope with diverse environmental conditions. Nitrate (NO3-) is the main source of nitrogen for plants and is a major component in fertilizer. Unraveling the molecular basis of nitrate sensing and regulation of nitrate uptake should enable the development of strategies to increase the efficiency of nitrogen use and maximize nitrate uptake by plants, which would aid in reducing nitrate pollution. NPF6.3 (also known as NRT1.1), which functions as a nitrate sensor and transporter; the kinase CIPK23; and the calcium sensor CBL9 form a complex that is crucial for nitrate sensing in Arabidopsis thaliana. We identified two additional components that regulate nitrate transport, sensing, and signaling: the calcium sensor CBL1 and protein phosphatase 2C family member ABI2, which is inhibited by the stress-response hormone abscisic acid. Bimolecular fluorescence complementation assays and in vitro kinase assays revealed that ABI2 interacted with and dephosphorylated CIPK23 and CBL1. Coexpression studies in Xenopus oocytes and analysis of plants deficient in ABI2 indicated that ABI2 enhanced NPF6.3-dependent nitrate transport, nitrate sensing, and nitrate signaling. These findings suggest that ABI2 may functionally link stress-regulated control of growth and nitrate uptake and utilization, which are energy-expensive processes.
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